BioMed Research International / 2014 / Article / Fig 4

Research Article

Anticancer Drug 2-Methoxyestradiol Protects against Renal Ischemia/Reperfusion Injury by Reducing Inflammatory Cytokines Expression

Figure 4

TUNEL staining of kidneys. Apoptotic cells in the kidneys of each group of mice were determined using TUNEL staining. All groups: . (a) There is no fluorescent intensity in the control kidney with saline treatment. (b) Apoptotic cells displaying high fluorescence intensity were detected in sections from I/R-only kidneys; however, (c) the green fluorescence of apoptotic nuclei is rare in tubular epithelial cells of the kidneys with 2ME2 (20 mg/kg) treatment. Sections were observed under a fluorescence microscope (×400). Scale bar is 50 μm. Representative data are shown. (d) Apoptotic cell counts in kidneys are averages of 10 high power fields (HPFs) from each group of mice. All groups: . compared with control mice and compared with I/R-only mice.