Anticancer Drug 2-Methoxyestradiol Protects against Renal Ischemia/Reperfusion Injury by Reducing Inflammatory Cytokines Expression
2ME2 reduced expression of HIF-1α, activated caspase-9, and pNF-κB but increased antiapoptotic BCL-xL and BCL-2. Expression levels of HIF-1α (a), pNF-κB (b), BCL-2 (c), BCL-xL (d), and activated caspase-9 (e) in kidneys were determined using western blotting in three groups: sham control: mice undergoing the same procedure without occlusion of the renal pedicle; I/R-only: mice given renal I/R challenge combined with vehicle (normal saline) treatment; I/R+2ME2: I/R-only mice injected with 2ME2 (10 mg/kg, i.p.). β-Actin was the internal control. Representative data are shown. All groups: . compared with control mice and compared with I/R-only mice.