Anticancer Drug 2-Methoxyestradiol Protects against Renal Ischemia/Reperfusion Injury by Reducing Inflammatory Cytokines Expression
2ME2 reduced ROS production and increased cell survival in antimycin-A (Aa)-treated renal cells. (a) Direct free radical scavenging activity of 2ME2 determined by the DPPH radical scavenging assay. Values represent the data of three independent experiments. Vitamin C was used as a positive control compound. (b) Cells were treated with antimycin-A 10 μM with saline or 2ME2 (2.5 or 5 μM) for 1 h. Quantitation of ROS generation in RMCs and NRK52E cells using CM-H2DCFDA assay. (c) RMCs and NRK52E cells were treated with antimycin-A 10 μM with saline or 2ME2 2.5 μM for 16 h. Cell survival was determined using MTT assay. All groups: . compared to untreated group and compared to antimycin-A group.