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BioMed Research International
Volume 2014 (2014), Article ID 438065, 9 pages
Research Article

Nanofibrous Chitosan-Polyethylene Oxide Engineered Scaffolds: A Comparative Study between Simulated Structural Characteristics and Cells Viability

1Department of Materials Science, Tampere University of Technology, P.O. Box 589, 33101 Tampere, Finland
2Department of Textile Engineering, Islamic Azad University South Tehran Branch, P.O. Box 11365-4435, Tehran, Iran
3Department of Pharmaceutical Chemistry, School of Pharmacy, Isfahan University of Medical Sciences, P.O. Box 81745-359, Isfahan, Iran
4VTT Technical Research Centre of Finland, P.O. Box 1000, 02044 VTT, Finland

Received 25 December 2013; Revised 7 April 2014; Accepted 8 May 2014; Published 4 June 2014

Academic Editor: Kibret Mequanint

Copyright © 2014 Mohammad Kazemi Pilehrood et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


3D nanofibrous chitosan-polyethylene oxide (PEO) scaffolds were fabricated by electrospinning at different processing parameters. The structural characteristics, such as pore size, overall porosity, pore interconnectivity, and scaffold percolative efficiency (SPE), were simulated by a robust image analysis. Mouse fibroblast cells (L929) were cultured in RPMI for 2 days in the presence of various samples of nanofibrous chitosan/PEO scaffolds. Cell attachments and corresponding mean viability were enhanced from 50% to 110% compared to that belonging to a control even at packed morphologies of scaffolds constituted from pores with nanoscale diameter. To elucidate the correlation between structural characteristics within the depth of the scaffolds’ profile and cell viability, a comparative analysis was proposed. This analysis revealed that larger fiber diameters and pore sizes can enhance cell viability. On the contrary, increasing the other structural elements such as overall porosity and interconnectivity due to a simultaneous reduction in fiber diameter and pore size through the electrospinning process can reduce the viability of cells. In addition, it was found that manipulation of the processing parameters in electrospinning can compensate for the effects of packed morphologies of nanofibrous scaffolds and can thus potentially improve the infiltration and viability of cells.