Exacerbated Skeletal Muscle Inflammation and Calcification in the Acute Phase of Infection by Mexican Trypanosoma cruzi DTUI Strain
Figure 4
Macrophages and calcification areas in skeletal muscle due to Querétaro strain infection. (a) Immunofluorescence of a large amastigote nest in skeletal muscle of mice infected with Querétaro strain, 21 days pi. (b) Same field showing immunofluorescence of macrophages surrounding the nest. (c) Merge of (a) and (b). Magnification 400x. (d) Microphotographs of amastigotes (green) inside a macrophage (red); counterstained with DAPI. Extreme right: merge. Zoom of a magnification 400x. (e) Calcification areas stained in brown (arrows), 21 days pi. (f) Calcification areas (arrows) at 90 days pi. The inserts show calcification areas stained with H-E. Magnification 100x.
