Exacerbated Skeletal Muscle Inflammation and Calcification in the Acute Phase of Infection by Mexican Trypanosoma cruzi DTUI Strain

Figure 5

Cytokines determination in skeletal muscle from mice infected with two Mexican Trypanosoma cruzi strains (Ninoa, cleared bars; Querétaro, solid bars; Control, stripped bars). mRNA expression levels were quantified by qPCR: (a) IFN-γ, (b) IL-4, (c) IL-12, (d) IL-10, and (e) TNF-α. Data are expressed as average ± SEM of 6 mice per experimental group from 2 independent experiments. ^★𝑃<0.05 with ANOVA test and Bonferroni posttest. (f) Cytokine concentration (pg/mL) of in vitro infections of macrophages J774 with each TcI strain. Data are presented as average ± SD of double measures from three independent experiments. ^★𝑃<0.05 with Student’s 𝑡-test.

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