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BioMed Research International
Volume 2014, Article ID 450621, 8 pages
http://dx.doi.org/10.1155/2014/450621
Research Article

RNA Sequencing Reveals Upregulation of RUNX1-RUNX1T1 Gene Signatures in Clear Cell Renal Cell Carcinoma

1Department of Urology, Huashan Hospital, Fudan University, 12 Central Urumqi Road, Shanghai 200040, China
2Fudan-VARI Center for Genetic Epidemiology, School of Life Sciences, Fudan University, Shanghai 200433, China
3Laboratory of Bioinformatics, Center for Genetic Epidemiology and Prevention, Van Andel Research Institute, 333 Bostwick Avenue NE, Grand Rapids, MI 49503, USA
4Center for Cancer Genomics, Wake Forest University School of Medicine, Medical Center Boulevard, Winston-Salem, NC 27157, USA
5Laboratory of Computational, Laboratory of Genomics and Prevention, Center for Genetic Epidemiology and Prevention, Van Andel Research Institute, 333 Bostwick Avenue NE, Grand Rapids, MI 49503, USA

Received 12 November 2013; Revised 21 January 2014; Accepted 31 January 2014; Published 25 March 2014

Academic Editor: Gary S. Stein

Copyright © 2014 Zuquan Xiong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the past few years, therapies targeted at the von Hippel-Lindau (VHL) and hypoxia-inducible factor (HIF) pathways, such as sunitinib and sorafenib, have been developed to treat clear cell renal cell carcinoma (ccRCC). However, the majority of patients will eventually show resistance to antiangiogenesis therapies. The purpose of our study was to identify novel pathways that could be potentially used as targets for new therapies. Whole transcriptome sequencing (RNA-Seq) was conducted on eight matched tumor and adjacent normal tissue samples. A novel RUNX1-RUNX1T1 pathway was identified which was upregulated in ccRCC through gene set enrichment analysis (GSEA). We also confirmed the findings based on previously published gene expression microarray data. Our data shows that upregulated of the RUNX1-RUNX1T1 gene set maybe an important factor contributing to the etiology of ccRCC.