BioMed Research International

Research Article

Experimental Measurements for the Effect of Dilution Procedure in Blood Esterases as Animals Biomarker for Exposure to OP Compounds

Table 2

Malathion point estimates (IC₂₀, IC₅₀, and IC₈₀; M) from concentration-inhibition curves in the presence of dilutions 1 : 5, 1 : 10, and 1 : 15. Two preincubation conditions were used: long (4 h) and short (1 h).


Preincubation condition	Slop	IC₂₀ (M)	Slop	IC₅₀ (M)	Slop	IC₈₀ (M)

Long (4 h)
Dilution (1 : 5)	0.22 ± 0.655^a	3.15 ± 0.991^a	0.67 ± 0.126	1.1 ± 0.432^a	0.66 ± 0.718	1.1 ± 0.121
Dilution (1 : 10)	1.4 ± 0.122	0.50 ± 0.654	0.89 ± 0.117	0.78 ± 0.121	0.64 ± 0.611	1.2 ± 0.211
Dilution (1 : 15)	0.11 ± 0.418^b	6.3 ± 1.2^b	0.71 ± 0.112	0.98 ± 0.212	0.84 ± 0.801	0.83 ± 0.221
Short (1 h)
Dilution (1 : 5)	0.34 ± 0.372^a	2.1 ± 0.876^a	0.29 ± 0.501^a	2.4 ± 0.311^a	0.37 ± 0.48^a	1.9 ± 0.421
Dilution (1 : 10)	0.66 ± 0.931	1.1 ± 0.765	0.27 ± 0.145	2.6 ± 0.911	0.29 ± 0.18	2.4 ± 0.987
Dilution (1 : 15)	0.12 ± 0.583^b	5.8 ± 1.1^b	0.53 ± 0.196^b	1.31 ± 0.876	0.24 ± 0.41^b	2.9 ± 0.766

Values in the table are the mean ± SE of three individual serum esterase (CbE) for quail. For the measurement of IC₂₀, IC₅₀, and IC₈₀, esterase enzyme was inhibited for 30 min at room temperature 25°C with appropriate concentrations for malathion compound. Then results were fitted with an exponential decay using SigmaPlot 11. ^a,bdenotes significant difference (analysis of variance (ANOVA), ) between dilution 1 : 5 and dilution 1 : 15.