BioMed Research International / 2014 / Article / Fig 2

Research Article

Differential Expression of Osteo-Modulatory Molecules in Periodontal Ligament Stem Cells in Response to Modified Titanium Surfaces

Figure 2

Osteogenic activity of periodontal ligament stem cells in response to titanium surfaces. Cells were cultured on pretreatment (PT), hydrophilic PT (pmodPT), sand-blasted, large-grit acid-etched (SLA), or hydrophilic SLA (modSLA) substrates for 4 or 7 days, and ALP activity ((a), (b)), real time RT-PCR ((c)–(f)), Western blot (g), and immunofluorescence staining (h) of osteogenic markers were then assessed as described in Section 2. Reported values are the means standard deviations of five independent experiments. Panels (bars) denote the means standard deviations of five experiments for each condition, determined from densitometry relative to -actin. versus control (tissue culture polystyrene); versus PT substrate. Nuclei were stained with DAPI (blue). A representative result from three independent experiments is shown.

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