Differential Expression of Osteo-Modulatory Molecules in Periodontal Ligament Stem Cells in Response to Modified Titanium Surfaces

Figure 4

Effect of 𝛽-catenin knockdown or NFAT inhibitor on integrins, Shh/Gli, and Notch/Hes-1 gene expression. mRNA expression levels of integrin 𝛼2, integrin 𝛽1, Shh, Gli1, Notch, and Hes-1 were analyzed after cells were transfected with 𝛽-catenin-specific siRNA for 48 h or treated with NFAT inhibitor VIVIT (500 nM). A representative result from four independent experiments is shown. ^∗𝑃<0.05 versus control (tissue culture polystyrene); ^#𝑃<0.05 versus pretreatment substrate; ^$𝑃<0.05 versus modified PT substrate; ^@𝑃<0.05 versus sand-blasted, large-grit acid-etched (SLA) substrate;^∗∗𝑃<0.05 versus modified SLA substrate.

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