Differential Expression of Osteo-Modulatory Molecules in Periodontal Ligament Stem Cells in Response to Modified Titanium Surfaces

Table 1

Primer sequences used for real-time RT-PCR analysis of gene expression.

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)
RUNX2	GTCTCACTGCCTCTCACTTG	CACACATCTCCTCCCTTCTG
OSX	TGAGGAGGAAGTTCACTATGG	TTCTTTGTGCCTGCTTTGC
FOSB	TCCAGGCGGAGACAGATCAGTTG	TCTTCGTAG GGGATCTTGCAGCC
FRA1	CCCTGCCGCCCTGTACCTTGTATC	AGACATTGGCTAGGGTGGCATCTGCA
Wnt3a	GTCCCGTCCCTCCCTTTC	ACCTCTCTTCCTACCTTTCCC
Wnt5a	TCTCAGCCCAAGCAACAAGG	GCCAGCATCACATCACAACAC
𝛽-catenin	GGCAGCAACAGTCTTACC	TCCACATCCTCTTCCTCA
Integrin 𝛼2	ACTGTTCAAGGAGGAGAC	GGTCAAAGGCTTGTTTAGG
Integrin 𝛽1	ATTACTCAGATCCAACCAC	TCCTCCTCATTTCATTCATC
Calmodulin	CAGATATTGATGGAGACGGA	GAGCACACGAAGTACAAGAG
NFATc1	CCTTCGGAAGGGTGCCTTTT	AGGCGTGGGGCCTCAGCAGG
Shh	CGCCAGCGGAAGGTATGAAG	CAACTTGTCCTTACACCTCTGAGTC
Gli1	AATGCTGCCATGGATGCTAGA	GAGTATCAGTAGGTGGGAAGTCCATAT
Notch	GCCGCCTTTGTGCTTCTGTTC	CCGGTGGTCTGTCTGGTCGTC
Hes-1	AGGCGGACATTCTGGAAATG	CGGTACTTCCCCAGCACACTT
GAPDH	GCTCTCCAGAACATCATCC	TGCTTCACCACCTTCTTG