Nuclear Nox4-Derived Reactive Oxygen Species in Myelodysplastic Syndromes

Figure 3

Western blot analysis of cytosol (cyto) and nuclear fractions (nuclei) of MOLM-13 and THP1 samples revealed with anti-Nox4, anti-Akt1, anti-ERK1/2, anti-Rac1, and anti-p22phox. Tubulin absence was used as index of nuclear extract’s purification. Presented data are representative of three independent experiments.