Nuclear Nox4-Derived Reactive Oxygen Species in Myelodysplastic Syndromes
Figure 4
Effect of Nox4 inhibition on nuclear ROS production. (a) Representative images showing staining with nuclear ROS probe (nuclear peroxy Emerald 1) of THP1 in the presence or absence of 2 μM DPI for 18 hours. Scale bar: 10 μm. (b) Graph representing fluorescence intensity of nuclear ROS probe (Nuclear peroxy Emerald 1) of MOLM-13 and THP1 in the presence or absence of 2 μM DPI for 18 hours. (c) Representative images showing: superimposing between DAPI (blue) and Nox4 SC (green) signals of THP1 treated with empty vector (EV) or Nox4-directed siRNA (38 and 40) as reported in Section 2. Scale bar: 10 μm. (d) Representative images of Western blot analysis of Nox4 silencing in THP1 cells. βactin was used as loading internal control. (e) Graph representing fluorescence intensity of nuclear ROS probe (Nuclear peroxy Emerald 1) of THP1 treated with empty vector (EV), scrambled siRNA (SCR), or Nox4-directed siRNA (37, 38, 39, and 40). Presented data are representative of three independent experiments. and versus Control.
