A Multiplex Snapback Primer System for the Enrichment and Detection of JAK2 V617F and MPL W515L/K Mutations in Philadelphia-Negative Myeloproliferative Neoplasms
Figure 6
Snapback primer assay identifying a patient DNA with MPL W515L mutation. (a) Peripheral blood DNA from a patient with PMF (J247) was detected as weak MPL W515L mutation positive . (b) Sanger sequencing of MPL W515L/K mutation after amplicon T-A cloning identified purified MPL W515L mutation clone. (c) Snapback primer assay discriminated the MPL W515L mutation from the wild-type control.
