Synthesis and Evaluation of Tetramethylguanidinium-Polyethylenimine Polymers as Efficient Gene Delivery Vectors
Figure 4
In vitro transfection assay. (a) Images of GFP expression after transfection with bPEI/pDNA and TP2/pDNA complexes in HEK293 cell at w/w ratio of 1.6 and 3.3, respectively. CHO, HEK293, and HeLa cells were transfected with pDNA complexes of TP polymers, bPEI, and Lipofectamine (LA) in the absence (b) and presence (c) of serum. Cells were incubated with complexes for 3 h and the expression was monitored after 36 h. The results represent the mean of three independent experiments performed in triplicate.
