Synthesis and Evaluation of Tetramethylguanidinium-Polyethylenimine Polymers as Efficient Gene Delivery Vectors

Figure 8

DNase I protection assay. TP2/pDNA complex, prepared at its best working w/w ratio of 3.3, was treated with 1 U of DNase I for 0.25, 0.5, 1.0, and 2.0 h. Complexes were treated with 20 U of heparin to release the pDNA from complex, which was run on a 0.8% agarose gel at 100 V for 45 min and stained with ethidium bromide. Released DNA was quantified densitometrically.