A Novel Promising Strain of Trichoderma evansii (WF-3) for Extracellular α-Galactosidase Production by Utilizing Different Carbon Sources under Optimized Culture Conditions
Table 2
Total protein (g/mL) secreted by T. evansii (WF-3) in the culture medium up to 11 days.
Culture media
Total protein (g/mL) secreted by T. evansii (WF-3) in the culture medium up to 11 days
Incubation time (days)
1
2
3
4
5
6
7
8
9
10
11
GG
164.57 ± 14.08
193.47 ± 15.09
136.60 ± 9.7
103.01 ± 9.8
182.16 ± 17.03
278.38 ± 26.08
265.07 ± 24.06
261.30 ± 25.09
160.80 ± 15.68
28.89 ± 2.69
18.56 ± 1.7
GG + GAL
266.33 ± 18.09
272.61 ± 20.08
321.60 ± 2.2
414.57 ± 33.06
582.91 ± 44.06
312.81 ± 29.85
296.48 ± 28.86
192.16 ± 18.49
159.80 ± 15.34
155.78 ± 14.68
26.89 ± 2.4
GG + SUC
95.47 ± 8.07
90.45 ± 7.6
41.45 ± 3.8
67.83 ± 5.9
35.17 ± 3.2
116.83 ± 10.5
329.15 ± 30.36
590.45 ± 58.78
419.59 ± 40.93
379.39 ± 36.90
331.71 ± 31.08
SM
435.92 ± 30.04
238.69 ± 19.06
314.07 ± 29.08
353.01 ± 30.08
798.24 ± 67.08
361.80 ± 34.05
328.15 ± 29.89
311.56 ± 30.65
291.45 ± 28.78
266.33 ± 25.08
150.01 ± 14.30
SM + GAL
182.16 ± 16.02
90.35 ± 7.8
229.89 ± 20.07
110.55 ± 10.9
239.94 ± 22.05
351.75 ± 34.02
79.15 ± 6.98
107.18 ± 9.89
174.62 ± 16.96
153.26 ± 14.90
160.80 ± 15.56
SM + SUC
207.28 ± 17.09
227.38 ± 20.04
189.69 ± 17.06
189.69 ± 17.68
340.45 ± 32.89
258.79 ± 23.83
242.46 ± 21.98
557.78 ± 53.09
518.84 ± 50.86
383.17 ± 37.68
535.17 ± 51.29
WS
67.83 ± 5.6
114.32 ± 10.89
145.72 ± 13.83
237.43 ± 22.87
329.15 ± 31.31
483.67 ± 46.76
463.56 ± 44.98
356.78 ± 32.88
341.71 ± 33.21
236.18 ± 22.26
230.46 ± 21.86
WS + GAL
10.05 ± 9.7
146.98 ± 13.89
145.30 ± 13.36
301.50 ± 29.76
146.98 ± 13.68
282.66 ± 27.60
214.82 ± 20.96
288.94 ± 27.27
417.08 ± 40.14
396.98 ± 38.36
160.80 ± 15.70
WS + SUC
43.96 ± 4.3
48.99 ± 4.3
74.12 ± 7.12
228.64 ± 21.75
271.35 ± 26.67
237.43 ± 22.80
194.72 ± 16.86
127.89 ± 11.87
635.67 ± 61.64
611.80 ± 60.68
569.78 ± 54.06
GAL
23.86 ± 2.2
67.83 ± 5.3
336.68 ± 32.39
395.72 ± 37.64
201.23 ± 19.63
37.40 ± 35.45
N/D
N/D
N/D
12.56 ± 1.1
N/D
SUC
23.14 ± 2.1
50.25 ± 4.8
60.30 ± 59.86
60.30 ± 5.9
37.68 ± 36.08
50.30 ± 4.9
115.57 ± 10.67
173.36 ± 15.46
152.01 ± 13.83
100.50 ± 9.78
231.46 ± 22.34
GG: guar gum, GG + GAL: guar gum + galactose, GG + SUC: guar gum + sucrose, SM: soya bean meal, SM + GAL: soya bean meal + galactose, SM + SUC: soya bean meal + sucrose, WS: wheat straw, WS + GAL: wheat straw + galactose, and WS + SUC: wheat straw + sucrose. The culture conditions were the same as described in Section 2. The experiments have been conducted three times and the values are shown as mean ± SD.
