A Novel Promising Strain of Trichoderma evansii (WF-3) for Extracellular α-Galactosidase Production by Utilizing Different Carbon Sources under Optimized Culture Conditions
Table 3
Effect of pH, incubation temperature, and substrate concentration on the level of total protein (g/mL) secreted by T. evansii (WF-3) in the culture filtrate.
Culture parameters
Culture media with different carbon sources
Total protein (g/mL) at 5th day of incubation
Total protein (g/mL) at 6th day of incubation
pH
3.5
GG + GAL
160.80 ± 15.07
173.36 ± 16.28
SM
87.93 ± 7.9
70.35 ± 6.8
WS + GAL
103.02 ± 9.8
86.68 ± 8.6
4.5
GG + GAL
105.52 ± 9.9
173.36 ± 15.89
SM
250.00 ± 24.9
133.17 ± 12.75
WS + GAL
246.23 ± 22.8
244.97 ± 23.9
5.5
GG + GAL
204.77 ± 19.7
139.45 ± 12.98
SM
62.81 ± 4.7
116.83 ± 15.43
WS + GAL
245.16 ± 24.09
247.53 ± 23.69
6.5
GG + GAL
223.61 ± 21.05
134.42 ± 11.78
SM
265.07 ± 25.08
164.57 ± 15.07
WS + GAL
360.55 ± 34.35
59.05 ± 55.98
7.4
GG + GAL
283.91 ± 25.86
38.53 ± 3.2
SM
114.32 ± 10.76
61.55 ± 5.9
WS + GAL
370.60 ± 35.68
209.79 ± 20.03
Substrate conc. (mg/mL)
6
GG + GAL
432.16 ± 42.14
203.52 ± 19.56
SM
104.27 ± 9.45
76.63 ± 6.8
WS + GAL
305.27 ± 29.67
82.91 ± 8.0
10
GG + GAL
476.13 ± 46.43
180.90 ± 16.68
SM
361.80 ± 34.09
457.28 ± 43.04
WS + GAL
146.98 ± 12.86
237.44 ± 22.14
14
GG + GAL
305.28 ± 28.05
80.40 ± 7.8
SM
193.46 ± 17.06
95.47 ± 8.8
WS + GAL
87.93 ± 7.6
38.94 ± 3.3
Incubation temperature (°C)
28
GG + GAL
192.16 ± 18.03
582.91 ± 54.24
SM
361.80 ± 35.64
798.24 ± 70.43
WS + GAL
146.98 ± 13.43
282.66 ± 23.87
40
GG + GAL
99.24 ± 9.3
193.49 ± 18.54
SM
31.40 ± 2.8
34.53 ± 3.3
WS + GAL
70.35 ± 6.4
82.35 ± 8.0
GG: guar gum, GG + GAL: guar gum + galactose, GG + SUC: guar gum + sucrose, SM: soya bean meal, SM + GAL: soya bean meal + galactose, SM + SUC: soya bean meal + sucrose, WS: wheat straw, WS + GAL: wheat straw + galactose, and WS + SUC: wheat straw + sucrose. The culture conditions were the same as described in Section 2. The experiments have been conducted three times and the values are shown as mean ± SD.
