Hypoxia-Pretreated Human MSCs Attenuate Acute Kidney Injury through Enhanced Angiogenic and Antioxidative Capacities
Effects of hypoxia preconditioning on the angiogenic and antioxidative capacities of MSCs in vitro and in kidney. (a) RT-PCR demonstrated that the transcription of angiogenic genes in MSCs was increased when cultured in hypoxia, while Elisa assay obtained consistent results from protein level; (b) DHE staining showed that hypoMSCs exhibited higher antioxidative activity than control MSCs after transplantation in I/R kidney; (c) angiogenic effects in I/R kidney by hypoMSCs. compared with normaxia or PBS group; compared with control MSCs. HypoMSC indicates hypoxia-preconditioned MSCs. Bar = 50 μm.