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BioMed Research International
Volume 2014 (2014), Article ID 465054, 9 pages
http://dx.doi.org/10.1155/2014/465054
Research Article

Molecular Screening of Virulence Genes in Extraintestinal Pathogenic Escherichia coli Isolated from Human Blood Culture in Brazil

Laboratory of Basic and Applied Bacteriology, Department of Microbiology, Universidade Estadual de Londrina, Rodovia Celso Garcia Cid, 86051-980 Londrina, PR, Brazil

Received 27 November 2013; Revised 13 March 2014; Accepted 17 March 2014; Published 15 April 2014

Academic Editor: Ketoki Kapila

Copyright © 2014 Vanessa L. Koga et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. H. S. Sader, R. N. Jones, S. Andrade-Baiocchi, and D. J. Biedenbach, “Four-year evaluation of frequency of occurrence and antimicrobial susceptibility patterns of bacteria from bloodstream infections in Latin American medical centers,” Diagnostic Microbiology and Infectious Disease, vol. 44, no. 3, pp. 273–280, 2002. View at Publisher · View at Google Scholar · View at Scopus
  2. K. B. Laupland, D. B. Gregson, D. L. Church, T. Ross, and J. D. D. Pitout, “Incidence, risk factors and outcomes of Escherichia coli bloodstream infections in a large Canadian region,” Clinical Microbiology and Infection, vol. 14, no. 11, pp. 1041–1047, 2008. View at Publisher · View at Google Scholar · View at Scopus
  3. O. Clermont, S. Bonacorsi, and E. Bingen, “Rapid and simple determination of the Escherichia coli phylogenetic group,” Applied and Environmental Microbiology, vol. 66, no. 10, pp. 4555–4558, 2000. View at Publisher · View at Google Scholar · View at Scopus
  4. M. Ananias and T. Yano, “Serogroups and virulence genotypes of Escherichia coli isolated from patients with sepsis,” Brazilian Journal of Medical and Biological Research, vol. 41, no. 10, pp. 877–883, 2008. View at Google Scholar · View at Scopus
  5. R. A. Conceição, M. S. Ludovico, C. G. T. J. Andrade, and T. Yano, “Human sepsis-associated Escherichia coli (SEPEC) is able to adhere to and invade kidney epithelial cells in culture,” Brazilian Journal of Medical and Biological Research, vol. 45, pp. 376–472, 2012. View at Publisher · View at Google Scholar
  6. G. Tomazetto, A. C. M. Silva, and M. C. Vidotto, “Fatores de virulência de amostras de Escherichia coli septicêmicas,” Biosaúde, vol. 6, pp. 3–16, 2004. View at Google Scholar
  7. M. Sabaté, E. Moreno, T. Pérez, A. Andreu, and G. Prats, “Pathogenicity island markers in commensal and uropathogenic Escherichia coli isolates,” Clinical Microbiology and Infection, vol. 12, no. 9, pp. 880–886, 2006. View at Publisher · View at Google Scholar · View at Scopus
  8. J. R. Johnson and A. L. Stell, “Extended virulence genotypes of Escherichia coli strains from patients with urosepsis in relation to phylogeny and host compromise,” The Journal of Infectious Diseases, vol. 181, no. 1, pp. 261–272, 2000. View at Publisher · View at Google Scholar · View at Scopus
  9. T. J. Johnson, Y. Wannemuehler, C. Doetkott, S. J. Johnson, S. C. Rosenberger, and L. K. Nolan, “Identification of minimal predictors of avian pathogenic Escherichia coli virulence for use as a rapid diagnostic tool,” Journal of Clinical Microbiology, vol. 46, no. 12, pp. 3987–3996, 2008. View at Publisher · View at Google Scholar · View at Scopus
  10. C. L. Le Bouguénec, M. Archambaud, and A. Labigne, “Rapid and specific detection of the pap, afa, and sfa adhesin-encoding operons in uropathogenic Escherichia coli strains by polymerase chain reaction,” Journal of Clinical Microbiology, vol. 30, no. 5, pp. 1189–1193, 1992. View at Google Scholar · View at Scopus
  11. C. I. Kado and S.-T. Liu, “Rapid procedure for detection and isolation of large and small plasmids,” Journal of Bacteriology, vol. 145, no. 3, pp. 1365–1373, 1981. View at Google Scholar · View at Scopus
  12. M. Z. Laporta, M. L. M. Silva, I. C. A. Scaletsky, and L. R. Trabulsi, “Plasmids coding for drug resistance and localized adherence to HeLa cells in Enteropathogenic Escherichia coli O55:H- and O55:H6,” Infection and Immunity, vol. 51, no. 2, pp. 715–717, 1986. View at Google Scholar · View at Scopus
  13. J. A. L. Sales Jr., C. M. David, R. Hatum et al., “Sepse Brasil: estudo epidemiológico da sepse em unidades de terapia intensiva brasileiras,” Revista Brasileira de Terapia Intensiva, vol. 18, pp. 9–17, 2006. View at Publisher · View at Google Scholar
  14. C.-D. Köhler and U. Dobrindt, “What defines extraintestinal pathogenic Escherichia coli?” International Journal of Medical Microbiology, vol. 301, no. 8, pp. 642–647, 2011. View at Publisher · View at Google Scholar · View at Scopus
  15. L. Skjot-Rasmussen, K. Ejrnaes, B. Lundgren, A. M. Hammerum, and N. Frimodt-Moller, “Virulence factors and phylogenetic grouping of Escherichia coli isolates from patients with bacteraemia of urinary tract origin relate to sex and hospital- vs. Community-acquired origin,” International Journal of Medical Microbiology, vol. 302, no. 3, pp. 129–134, 2012. View at Publisher · View at Google Scholar · View at Scopus
  16. A. Ostblom, I. Adlerberth, A. E. Wold, and F. L. Nowrouzian, “Pathogenicity island markers, virulence determinants malX and usp, and the capacity of Escherichia coli to persist in infants' commensal microbiotas,” Applied and Environmental Microbiology, vol. 77, no. 7, pp. 2303–2308, 2011. View at Publisher · View at Google Scholar · View at Scopus
  17. S. Schubert, A. Rakin, and J. Heesemann, “The Yersinia high-pathogenicity island (HPI): evolutionary and functional aspects,” International Journal of Medical Microbiology, vol. 294, no. 2-3, pp. 83–94, 2004. View at Publisher · View at Google Scholar · View at Scopus
  18. B. Middendorf, B. Hochhut, K. Leipold, U. Dobrindt, G. Blum-Oehler, and J. Hacker, “Instability of Pathogenicity Islands in Uropathogenic Escherichia coli 536,” Journal of Bacteriology, vol. 186, no. 10, pp. 3086–3096, 2004. View at Publisher · View at Google Scholar · View at Scopus
  19. F. Bert, B. Huynh, F. Dondero et al., “Molecular epidemiology of Escherichia coli bacteremia in liver transplant recipients,” Transplant Infectious Disease, vol. 13, no. 4, pp. 359–365, 2011. View at Publisher · View at Google Scholar · View at Scopus
  20. A. F. P. Sonnem and P. Henneke, “Role of pore-forming toxins in neonatal sepsis,” Clinical and Developmental Immunology, vol. 2013, Article ID 608456, 13 pages, 2013. View at Publisher · View at Google Scholar
  21. J. Bien, O. Sokolova, and P. Bozko, “Role of uropathogenic Escherichia coli virulence factors in development of urinary tract infection and kidney damage,” International Journal of Nephrology, vol. 2012, Article ID 681473, 15 pages, 2012. View at Publisher · View at Google Scholar
  22. E. Moreno, I. Planells, G. Prats, A. M. Planes, G. Moreno, and A. Andreu, “Comparative study of Escherichia coli virulence determinants in strains causing urinary tract bacteremia versus strains causing pyelonephritis and other sources of bacteremia,” Diagnostic Microbiology and Infectious Disease, vol. 53, no. 2, pp. 93–99, 2005. View at Publisher · View at Google Scholar · View at Scopus
  23. G. Otto, M. Magnusson, M. Svensson, J. H. Braconier, and C. Svanborg, “pap Genotype and P fimbrial expression in Escherichia coli causing bacteremic and nonbacteremic febrile urinary tract infection,” Clinical Infectious Diseases, vol. 32, no. 11, pp. 1523–1531, 2001. View at Publisher · View at Google Scholar · View at Scopus
  24. A. Garénaux, M. Caza, and C. M. Dozois, “The Ins and Outs of siderophore mediated iron uptake by extra-intestinal pathogenic Escherichia coli,” Veterinary Microbiology, vol. 153, no. 1-2, pp. 89–98, 2011. View at Publisher · View at Google Scholar · View at Scopus
  25. Q. Gao, X. Wang, H. Xu et al., “Roles of iron acquisition systems in virulence of extraintestinal pathogenic Escherichia coli: salmochelin and aerobactin contribute more to virulence than heme in a chicken infection model,” BMC Microbiology, vol. 143, pp. 1–12, 2012. View at Google Scholar
  26. F. Hilali, R. Ruimy, P. Saulnier et al., “Prevalence of virulence genes and clonality in Escherichia coli strains that cause bacteremia in cancer patients,” Infection and Immunity, vol. 68, no. 7, pp. 3983–3989, 2000. View at Publisher · View at Google Scholar · View at Scopus
  27. J. R. Johnson and T. A. Russo, “Uropathogenic Escherichia coli as agents of diverse non-urinary tract extraintestinal infections,” The Journal of Infectious Diseases, vol. 186, no. 6, pp. 859–864, 2002. View at Publisher · View at Google Scholar · View at Scopus
  28. D. Mokady, U. Gophna, and E. Z. Ron, “Extensive gene diversity in septicemic Escherichia coli strains,” Journal of Clinical Microbiology, vol. 43, no. 1, pp. 66–73, 2005. View at Publisher · View at Google Scholar · View at Scopus
  29. P. Bidet and B. E. Bingen, “Facteurs de pathogénicité et physiopathologie des Escherichia coli extra-intestinaux,” Archives de Pédiatrie, vol. 19, pp. S80–S92, 2012. View at Publisher · View at Google Scholar
  30. J. R. Johnson, O. Clermont, M. Menard, M. A. Kuskowski, B. Picard, and E. Denamur, “Experimental mouse lethality of Escherichia coli isolates, in relation to accessory traits, phylogenetic group, and ecological source,” The Journal of Infectious Diseases, vol. 194, no. 8, pp. 1141–1150, 2006. View at Publisher · View at Google Scholar · View at Scopus
  31. D. Pérez-Mendoza and F. de la Cruz, “Escherichia coli genes affecting recipient ability in plasmid conjugation: are there any?” BMC Genomics, vol. 10, article 71, 2009. View at Publisher · View at Google Scholar · View at Scopus
  32. C. Lemaître, F. Mahjoub-Messai, D. Dupont et al., “A conserved virulence plasmidic region contributes to the virulence of the multiresistant Escherichia coli meningitis strain S286 belonging to phylogenetic group C,” PLOS ONE, vol. 8, no. 9, Article ID e74423, 2013. View at Google Scholar
  33. R. K. T. Kobayashi, I. Aquino, A. L. D. S. Ferreira, and M. C. Vidotto, “EcoR phylogenetic analysis and virulence genotyping of avian pathogenic Escherichia coli strains and Escherichia coli isolates from commercial chicken carcasses in southern Brazil,” Foodborne Pathogens and Disease, vol. 8, no. 5, pp. 631–634, 2011. View at Publisher · View at Google Scholar · View at Scopus
  34. M. Blanco, J. E. Blanco, M. P. Alonso, and J. Blanco, “Virulence factors and O 277 groups of Escherichia coliisolates from patients with acute pyelonephritis, cystitis, 278 and asymptomatic bacteriuria,” European Journal of Epidemiology, vol. 12, pp. 191–198, 1996. View at Google Scholar