Molecular Screening of Virulence Genes in Extraintestinal Pathogenic Escherichia coli Isolated from Human Blood Culture in Brazil

Table 1

Primers for detection of PAIs markers, phylogenetic analysis, virulence genes, and their respective virulence factors.

Genes	Sequence (5′ to 3′)	Size of product (bp)	Virulence factors	Reference
PAI I 536	TAA TGC CGG AGA TTC ATT GTC; AGG ATT TGT CTC AGG GCT TT	1.800	𝛼-Haemolysin, CS12 fimbriae, and F17-like fimbrial adhesin	Sabaté et al., 2006 [7]
PAI II 536	CAT GTC CAA AGC TCG AGC C; CTA CGT CAG GCT GGC TTT G	1.000	𝛼-Haemolysin and P-related fimbriae	Sabaté et al., 2006 [7]
PAI IV 536	AAG GAT TCG CTG TTA CCG GAC; TCG TCG GGC AGC GTT TCT TCT	300	Yersiniabactin siderophore system	Sabaté et al., 2006 [7]
PAI I CFT073	GGA CAT CCT GTT ACA GCG CGC A; TCG CCA CCA ATC ACA GC GAA C	930	𝛼-Haemolysin, P-fimbriae, and aerobactin	Sabaté et al., 2006 [7]
PAI II CFT073	ATG GAT GTT GTA TCG CGC; ACG AGC ATG TGG ATC TGC	400	P-fimbriae and iron-regulated genes	Sabaté et al., 2006 [7]
PAI I J96	TCG TGC TCA GGT CCG GAA TTT; TGG CAT CCC ACA TTA TCG	400	𝛼-Haemolysin and P-fimbriae	Sabaté et al., 2006 [7]
PAI II J96	GGA TCC ATG AAA ACA TGG TTA ATG GG; GAT ATT TTT GTT GCC ATT GGT TAC C	2.300	𝛼-Haemolysin, Prs-fimbriae, and cytotoxic necrotizing factor 1	Sabaté et al., 2006 [7]
chuA	GAC GAA CCA ACG GTC AGG AT; TGC CGC CAG TAC CAA AGA CA	279	Hemetransport in enterohemorrhagic O157:H7 E. coli	Clermont et al., 2000 [3]
yjaA	TGA AGT GTC AGG AGA CGC TG; ATG GAG AAT GCG TTC CTC AAC	211	Protein of function unknown	Clermont et al., 2000 [3]
TSPE4.C2	GAG TAA TGT CGG GGC ATT CA; CGC GCC AAC AAA GTA TTA CG	152	Putative DNA fragment (TSPE4.C2) in E. coli	Clermont et al., 2000 [3]
kpsII	GCG CAT TTG CTG ATA CTG TTG; CAT CCA GAC GAT AAG CAT GAG CA	272	Capsule synthesis K1 e K5	Johnson and Stell, 2000 [8]
K1	TAG CAA ACG TTC TAT TGG TGC; CAT CCA GAC GAT AAG CAT GAG CA	156	Capsule K1	Johnson and Stell, 2000 [8]
cvaC	CAC ACA CAA ACG GGA GCT GTT; CTT CCC GCA GCA TAG TTC CAT	680	Colicin V	Johnson and Stell, 2000 [8]
iutA	GGC TGG ACA TCA TGG GAA CTG G; CGT CGG GAA CGG GTA GAA TCG	302	Aerobactin siderophore receptor	Johnson et al., 2008 [9]
fyuA	TGA TTA ACC CCG CGA CGG AA; CGC AGT AGG CAC GAT CTT GTA	880	Yersiniabactin	Johnson and Stell, 2000 [8]
papC	GAC GGC TGT ACT GCA GGG TGT GGC G; ATA TCC TTT CTG CAG GCA GGG TGT GGC	328	P fimbriae	Le Bouguénec et al., 1992 [10]
papG	CTG TAA TTA CGG AAG TGA TTT CTG; ACT ATC CGG CTC CGG ATA AAC CAT	1070	P fimbriae	Johnson and Stell, 2000 [8]
sfaA	CTC CGG AGA ACT GGG TGC ATC TTA C; CGG AGG AGT AAT TAC AAA CCT GGC A	410	Sfa fimbriae	Le Bouguénec et al., 1992 [10]
sfaS	GTG GAT ACG ACG ATT ACT GTG; CCG CCA GCA TTC CCT GTA TTC	240	Sfa fimbriae	Johnson and Stell, 2000 [8]
Afa	GGC AGA GGG CCG GCA ACA GGC; CCC GTA ACG CGC CAG CAT CTC	559	M fimbriae	Johnson and Stell, 2000 [8]
ibe10	AGG CAG GTG TGC GCC GCG TAC; TGG TGC TCC GGC AAA CCA TGC	170	Invasion of brain endothelium	Johnson and Stell, 2000 [8]
hlyA	AAC AAG GAT AAG CAC TGT TCT GGC; ACC ATA TAA GCG GTC ATT CCC GTC	1.177	Hemolysin	Johnson and Stell, 2000 [8]
cnf1	AGG ATG GAG TTT CCT ATG CAG GAG; CAT TCA GAG TCC TGC CCT CAT TAT T	498	Cytotoxic necrotizing factor 1	Johnson and Stell, 2000 [8]
cnf2	AAT CTA ATT AAA GAG AAC; CAT GCT TTG TAT ATC TA	543	Cytotoxic necrotizing factor 2	Blanco et al., 1996 [34]
traT	GGT GTG GTG CGA TGA GCA CAG; CAC GGT TCA GCC ATC CCT GAG	290	Serum resistance	Johnson and Stell, 2000 [8]
iroN	AAT CCG GCA AAG AGA CGA ACC GCC T; GTT CGG GCA ACC CCT GCT TTG ACT TT	553	Salmochelin siderophore receptor	Johnson et al., 2008 [9]
ompT	TCA TCC CGG AAG CCT CCC TCA CTA CTA T; TAG CGT TTG CTG CAC TGG CTT CTG ATA C	496	Episomal outer membrane protease	Johnson et al., 2008 [9]
hlyF	GGC CAC AGT CGT TTA GGG TGC TTA CC; GGC GGT TTA GGC ATT CCG ATA CTC AG	450	Putative avian hemolysin	Johnson et al., 2008 [9]
Iss	CAG CAA CCC GAA CCA CTT GAT G; AGC ATT GCC AGA GCG GCA GAA	323	Episomal increased serum survival	Johnson et al., 2008 [9]