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BioMed Research International
Volume 2014 (2014), Article ID 467465, 10 pages
http://dx.doi.org/10.1155/2014/467465
Research Article

Punicalagin and Ellagic Acid Demonstrate Antimutagenic Activity and Inhibition of Benzo[a]pyrene Induced DNA Adducts

1Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh 202002, India
2James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA
3Department of Pharmacology and Toxicology, University of Louisville, Louisville, KY 40202, USA
4Department of Medicine, University of Louisville, Delia Baxter II, Room 304B, 580 S. Preston Street, Louisville, KY 40202, USA

Received 1 March 2014; Accepted 22 April 2014; Published 14 May 2014

Academic Editor: Davor Zeljezic

Copyright © 2014 Maryam Zahin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Punicalagin (PC) is an ellagitannin found in the fruit peel of Punica granatum. We have demonstrated antioxidant and antigenotoxic properties of Punica granatum and showed that PC and ellagic acid (EA) are its major constituents. In this study, we demonstrate the antimutagenic potential, inhibition of BP-induced DNA damage, and antiproliferative activity of PC and EA. Incubation of BP with rat liver microsomes, appropriate cofactors, and DNA in the presence of vehicle or PC and EA showed significant inhibition of the resultant DNA adducts, with essentially complete inhibition (97%) at 40 μM by PC and 77% inhibition by EA. Antimutagenicity was tested by Ames test. PC and EA dose-dependently and markedly antagonized the effect of tested mutagens, sodium azide, methyl methanesulfonate, benzo[a]pyrene, and 2-aminoflourine, with maximum inhibition of mutagenicity up to 90 percent. Almost all the doses tested (50–500 μM) exhibited significant antimutagenicity. A profound antiproliferative effect on human lung cancer cells was also shown with PC and EA. Together, our data show that PC and EA are pomegranate bioactives responsible for inhibition of BP-induced DNA adducts and strong antimutagenic, antiproliferative activities. However, these compounds are to be evaluated in suitable animal model to assess their therapeutic efficacy against cancer.