Punicalagin and Ellagic Acid Demonstrate Antimutagenic Activity and Inhibition of Benzo[a]pyrene Induced DNA Adducts
Figure 2
(a) Representative autoradiographs of 32P-postlabelling analysis of microsomal-benzo[a]pyrene (BP) DNA adducts in the presence of vehicle alone (2% DMSO), BP (1 μM) + vehicle, BP (1 μM) + punicalagin (PC) (40 μM), and BP (1 μM) + ellagic acid (EA) (40 μM). Adduct 1, anti-benzo[a]pyrene-7,8-diol-9,10-epoxide-dG, and adduct 2, 9-OH-benzo[a]pyrene-4,5-epoxide-dG. PC and EA alone group were not included since we do not expect any background BP-DNA adduct. OR, origin. (b) Inhibition of microsomal BP-induced DNA adducts by PC and EA. DNA adducts were analyzed by 32P-postlabeling assay. Data represent an average ± standard error of 4–6 samples. ; . Part of the figure is reprinted from Mutat. [28] Copyright (2014), with permission from Elsevier.
