Punicalagin and Ellagic Acid Demonstrate Antimutagenic Activity and Inhibition of Benzo[a]pyrene Induced DNA Adducts

Table 5

Effect of ellagic acid on the 2-aminofluorene induced mutagenicity in Salmonella typhimurium.

Treatment	Dose (μM)	Number of His^+ revertants colonies/plate (mean ± SE)
		TA 97a	TA 98	TA 100	TA 102
Spontaneous		144.0±8.7	39.7±3.8	140.3±10.8	325.7±16.6
Positive control (2AF)	1.5 μg	336.7±14.4	254.0±11.8	510.3±23.9	1475.0±38.1
^aEllagic acid	50	142.3±10.7	50.0±5.1	136.3±12.0	336.0±23.0
	100	159.7±10.5	44.7±3.8	162.7±16.6	275.0±16.8
	250	175.0±15.0	38.3±2.3	178.0±12.7	308.0±17.2
	500	184.0±10.6	32.0±3.6	188.7±12.8	302.0±17.1
^bEllagic acid + 2AF	50	312.3±20.5 (12.5)	214.3±13.9 (19.4)	426.0±18.6^∗ (22.5)	1276.0±38.0^∗ (17.5)
	100	296.7±13.4 (22.6)	171.0±9.5^∗∗ (39.6)	350.0±12.8^∗∗ (46.1)	924.0±25.3^∗∗∗ (45.9)
	250	264.0±14.2^∗ (44.9)	132.7±9.3^∗∗ (56.3)	285.3±13.1^∗∗ (67.7)	610.0±26.2^∗∗∗ (74.1)
	500	212.0±10.4^∗∗ (81.7)	76.3±6.1^∗∗∗ (80.0)	240.7±9.8^∗∗∗ (83.8)	431.0±13.6^∗∗∗ (89.0)
𝑅^2		0.93	0.99	0.99	0.98

aNegative control; ^bpreincubation test; values in parenthesis are % inhibition of mutagenicity.
^∗𝑃<0.05; ^∗∗𝑃<0.005 and ^∗∗∗𝑃<0.001; 2AF: 2-aminofluorene; 𝑅²: linear regression analysis.