Punicalagin and Ellagic Acid Demonstrate Antimutagenic Activity and Inhibition of Benzo[a]pyrene Induced DNA Adducts

Table 6

Effect of ellagic acid on the benzo[a]pyrene induced mutagenicity in Salmonella typhimurium.

Treatment	Dose (μM)	Number of His^+ revertants colonies/plate (mean ± SE)
		TA 97a	TA 98	TA 100	TA 102
Spontaneous		144.0±8.7	39.7±3.8	140.3±10.8	325.7±16.6
Positive control (BP)	1.5 μg	736.7±30.1	165.7±10.2	704.3±27.0	694.0±20.8
^aEllagic acid	50	142.3±10.7	50.0±5.1	136.3±12.0	336.0±23.0
	100	159.7±10.5	44.7±3.8	162.7±16.6	275.0±16.8
	250	175.0±15.0	38.3±2.3	178.0±12.7	308.0±17.2
	500	184.0±10.6	32.0±3.6	188.7±12.8	302.0±17.1
^bEllagic acid + BP	50	652.7±35.3 (14.1)	140.0±12.2 (22.2)	580.3±25.6^∗ (21.8)	653.0±34.7 (11.5)
	100	529.0±28.6^∗∗ (36.0)	107.3±7.9^∗ (48.2)	472.3±19.4^∗∗ (42.8)	595.0±24.1^∗ (23.6)
	250	333.3±22.2^∗∗∗ (71.8)	86.3±5.8^∗∗ (62.3)	336.0±17.6^∗∗∗ (70.0)	514.0±21.4^∗∗ (46.6)
	500	275.3±17.5^∗∗∗ (83.5)	60.7±10.5^∗∗ (78.6)	245.7±14.4^∗∗∗ (88.9)	366.0±25.0^∗∗∗ (83.7)
𝑅^2		098	0.97	0.99	0.93

aNegative control; ^bpreincubation test; values in parenthesis are % inhibition of mutagenicity.
^∗𝑃<0.05; ^∗∗𝑃<0.005 and ^∗∗∗𝑃<0.001; B[a]P: benzo[a]pyrene; 𝑅²: linear regression analysis.