Induction of Apoptosis of 2,4′,6-Trihydroxybenzophenone in HT-29 Colon Carcinoma Cell Line
Figure 6
(a) Effect of 2,4′,6-trihydroxy-4-methoxybenzophenone on HT-29 cell cycle. Cells were treated with the IC50 value (30 µM) concentrations of compound for 24 h, 48 h, and 72 h and analyzed by flow cytometry after staining with PI. Percentages of diploid cells (DNA content) at G0/G1, S, and G2/M phases of HT-29 cells were determined after 24 h, 48 h, and 72 h incubation periods. (b) Histogram showing quantitative percentage of diploid cells (DNA content) in each cell cycle phase without treatment and with treatment. (c) The expression of the whole protein in Coomassie blue staining after compound induced apoptosis in HT-29 cells. (d) The expression of antiapoptotic protein and proapoptotic protein in western blotting analysis.
