BioMed Research International / 2014 / Article / Fig 1

Research Article

Lung Cancer Stem Cell Lose Their Stemness Default State after Exposure to Microgravity

Figure 1

(a) Formation of H460 spheroids cultured in nonadherent conditions at the first and at the second generation after 24–48 h of seeding. (b) Spheroids-forming assay was carried out at 6–24 and 48 h of RPM-exposure and spheroids were counted by optical microscopy. Results represent mean ± SD (). Student’s -test, . (c) Representative spheroids obtained from H460 cells on ground or RPM condition at 0–6 and 24 h. (d) H460 spheroids exposed to RPM and then cultured on ground for 24 and 48 h. H460 spheroids give rise to de novo irregular cell aggregates.

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