Pinocembrin Protects Human Brain Microvascular Endothelial Cells against Fibrillar Amyloid- β₁₋₄₀ Injury by Suppressing the MAPK/NF- κ B Inflammatory Pathways

Figure 2

Cytoprotective effects of pinocembrin on hBMECs against fAβ _1–40-induced toxicity. (a) Pinocembrin increases cell viability as evaluated by MTS assay. (b) Pinocembrin decreases the levels of extracellular LDH released from hBMECs against fAβ _1–40-induced toxicity. (c) Representative images of nuclei stained by Hoechst 33342. Pinocembrin attenuates nuclear damage in hBMECs in the presence of fAβ _1–40 (×20). (d) Pinocembrin inhibits nuclear mean fluorescence intensity in hBMECs against fAβ _1–40-induced toxicity. Data are expressed as means ± SEM, 𝑛=6, ^∗∗∗𝑃<0.001 versus control, ^#𝑃<0.05, ^##𝑃<0.01, ^###𝑃<0.001 versus fAβ _1–40.

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