BioMed Research International / 2014 / Article / Fig 3

Research Article

Pinocembrin Protects Human Brain Microvascular Endothelial Cells against Fibrillar Amyloid- β1−40 Injury by Suppressing the MAPK/NF- κ B Inflammatory Pathways

Figure 3

Effects of pinocembrin on redox imbalance of hBMECs against fAβ 1–40-induced toxicity. (a) Representative images of intracellular ROS stained by DCFH2-DA. Pinocembrin cannot decrease the generation of ROS in hBMECs in the presence of fAβ 1–40 (×20). (b) Intracellular mean fluorescence intensity was assessed based on the DCF fluorescence on the ArrayScan HCS Reader with the Morphology Explorer BioApplication. Pinocembrin does not decrease mean fluorescence intensity in hBMECs against fAβ 1–40-induced toxicity. (c) Pinocembrin does not improve the SOD activity in hBMECs against fAβ 1–40-induced toxicity. (d) Pinocembrin does not increase the GSH-Px activity in hBMECs against fAβ 1–40-induced toxicity. Data are expressed as means ± SEM, , versus control.
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