Pinocembrin Protects Human Brain Microvascular Endothelial Cells against Fibrillar Amyloid-
Injury by Suppressing the MAPK/NF-
B Inflammatory Pathways
Effects of pinocembrin on the NF-κB signal activation and the release of proinflammatory cytokines of hBMECs against fAβ 1–40-induced toxicity. (a) Representative immunoblots for phosphor-IKKα, IKKα, phosphor-IKKβ, IKKβ, and IκBα in hBMECs extracts. (b) Quantitative analysis of these proteins. (d) Images of NF-κB p65 translocation from cytoplasm to nucleus analyzed on the ArrayScan HCS Reader (×20). (e) The value of Mean_CircRingAvgIntenDiff describing the translocation capacity of cytosolic phospho-p65 to the nucleus. (f), (g), and (h) Levels of TNF-α, IL-1β and IL-6 in hBMECs culture supernatant after exposure to fAβ 1–40. Data are expressed as means ± SEM, , versus control, , versus fAβ 1–40.