BioMed Research International / 2014 / Article / Fig 5

Research Article

Pinocembrin Protects Human Brain Microvascular Endothelial Cells against Fibrillar Amyloid- β1−40 Injury by Suppressing the MAPK/NF- κ B Inflammatory Pathways

Figure 5

Effects of pinocembrin on the NF-κB signal activation and the release of proinflammatory cytokines of hBMECs against fAβ 1–40-induced toxicity. (a) Representative immunoblots for phosphor-IKKα, IKKα, phosphor-IKKβ, IKKβ, and IκBα in hBMECs extracts. (b) Quantitative analysis of these proteins. (d) Images of NF-κB p65 translocation from cytoplasm to nucleus analyzed on the ArrayScan HCS Reader (×20). (e) The value of Mean_CircRingAvgIntenDiff describing the translocation capacity of cytosolic phospho-p65 to the nucleus. (f), (g), and (h) Levels of TNF-α, IL-1β and IL-6 in hBMECs culture supernatant after exposure to fAβ 1–40. Data are expressed as means ± SEM, , versus control, , versus fAβ 1–40.
470393.fig.005a
(a)
470393.fig.005b
(b)
470393.fig.005c
(c)
470393.fig.005d
(d)
470393.fig.005e
(e)
470393.fig.005f
(f)
470393.fig.005g
(g)
470393.fig.005h
(h)