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BioMed Research International
Volume 2014, Article ID 470425, 6 pages
http://dx.doi.org/10.1155/2014/470425
Research Article

Prophylactic Efficacy of Melatonin on Cyclophosphamide-Induced Liver Toxicity in Mice

1Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Mazandaran University of Medical Sciences, 18 Kilometers of Farah Abad Road, Sari 48175-861, Iran
2Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, 18 Kilometers of Farah Abad Road, Sari 48175-861, Iran
3Department of Pathology, Faculty of Medicine, Mazandaran University of Medical Sciences, 18 Kilometers of Farah Abad Road, Sari 48175-861, Iran
4Student Research Committee, Mazandaran University of Medical Sciences, 18 Kilometers of Farah Abad Road, Sari 48175-861, Iran

Received 12 February 2014; Accepted 13 June 2014; Published 30 June 2014

Academic Editor: Masood Ahmad

Copyright © 2014 Mohammad Shokrzadeh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The current study aimed to evaluate the protective effects of melatonin, a pineal secretory product, against hepatotoxicity induced by cyclophosphamide (CP) in mice. Mice were pretreated with melatonin intraperitoneally for 7 consecutive days before the administration of a single intraperitoneal dose of 200 mg/kg CP. 24 hr after CP administration, the mice were anesthetized, blood was then removed, and serum toxicity enzymes activities were evaluated. After the blood sampling, all animals were killed, livers were then removed, and histological studies were conducted. Serum toxicity marker enzymes were significantly increased after CP treatment but restored in melatonin pretreated groups. In addition, administration of CP induced necrotic hepatocyte with small crushed nuclei, portal space with severe inflammation, and hepatocytes surrounded by lymphocytic infiltration in hepatic tissues. However, melatonin effectively protected against CP-induced histopathological abnormalities in the liver tissues. Our results reveal that melatonin produces a potent hepatoprotective mechanism against CP. Therefore, melatonin could be a potent candidate to use concomitantly as a supplement agent against hepatotoxicity of CP for the patients undergoing chemotherapy.