The Putative HORMA Domain Protein Atg101 Dimerizes and Is Required for Starvation-Induced and Selective Autophagy in Drosophila
Figure 7
Atg13 domain structure and fragments used in mapping experiments [9] (a). Atg101-FLAG coprecipitates the full-length HA-Atg13, and also HA-GFP-Atg13 fragments containing the N-terminal HORMA domain: 1–230 and 1–398, but not the middle (231–398) or C-terminal (393–523) Atg13 fragments (b). Atg101-FLAG coprecipitates with HA-Atg101, and HA-Atg101 coprecipitates with both FLAG-Atg101 and Atg101-FLAG, but not with anti-HA or anti-FLAG beads alone, respectively (c).
