Age Modulates Fe3O4 Nanoparticles Liver Toxicity: Dose-Dependent Decrease in Mitochondrial Respiratory Chain Complexes Activities and Coupling in Middle-Aged as Compared to Young Rats
Figure 5
Effects of 350 μg/mL of Fe3O4 but not in its particulate form on middle-aged liver mitochondrial respiration and coupling. (a) reflects complexes I, III, and IV activities and is measured using glutamate and malate. (b) reflects complexes II, III, and IV activities and is measured using succinate. (c) reflects complex IV activity and is measured using N, N, N′, N′-tetramethyl-p-phenylenediaminedihydrochloride (TMPD) and ascorbate as mitochondrial substrates. (d) Acceptor control ratio reflects the mitochondrial coupling. Data are means ± SEM.
