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BioMed Research International
Volume 2014, Article ID 474616, 12 pages
http://dx.doi.org/10.1155/2014/474616
Research Article

Investigation of Antiarthritic Potential of Plumeria alba L. Leaves in Acute and Chronic Models of Arthritis

1Institute of Pharmaceutical Sciences, Kurukshetra University, Kurukshetra, Haryana 136119, India
2Department of Pharmacy, School of Chemical Science and Pharmacy, Central University of Rajasthan, Ajmer 305801, India
3Dabur Research and Development Centre, Ghaziabad 201002, India
4All India Institute of Medical Sciences, New Delhi 110029, India

Received 24 February 2014; Accepted 18 May 2014; Published 15 June 2014

Academic Editor: Shigeru Kotake

Copyright © 2014 Manjusha Choudhary et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. The present investigation was designed to evaluate antiarthritic potential of fractions of hydroalcoholic extract from leaves of P. alba. Materials and Methods. Plumeria alba L. leaves were extracted with hydroalcohol (30 : 70) to obtain hydroalcoholic extract of P. alba. This extract was further fractionated with solvents ethyl acetate and n-butanol to obtain EAPA and BPA, respectively. These fractions were tested against formaldehyde and Freund’s complete adjuvant (FCA) induced arthritis. Arthritis assessment, paw volume, body weight, motor incoordination, and nociceptive threshold were measured. On day 21, the animals were sacrificed and histopathology was done. Results. The 100 and 200 mg/kg doses of EAPA and BPA caused a significant () reduction in paw swelling in both models. Erythrocyte sedimentation rate (ESR) and spleen weight decreased significantly () in arthritic rats treated with extracts. There was significant () improvement in thymus weight in EAPA treated rats whereas significant () improvement was also seen in haemoglobin level (Hb) in diclofenac treated group. Motor incoordination and nociceptive threshold were also significantly () improved. Conclusion. The present study suggests that Plumeria alba L. has protective activity against arthritis and supports the traditional use of P. alba for rheumatism and other inflammatory diseases.