Antiproliferative and Apoptotic Effects of Sesbania grandiflora Leaves in Human Cancer Cells
Figure 7
DCFDA staining for ROS generation of human lung cancer cells A549 in the presence or absence of the methanolic fraction of S. grandiflora leaves. (a) Untreated A549 cells. (b) A549 cells treated with IC50 dose of the methanolic fraction for 24 h. (c) Spectrofluorometer measurement for the levels of ROS intermediates in untreated A549 cells. (d) Spectrofluorometer measurement for the levels of ROS intermediates in treated A549 cells. (e) Densitometry graph comparing the levels of ROS intermediates in untreated and treated A549 cells. Values are mean S.D from three independent experiments. ANOVA followed by Tukey’s test was used to assess the statistical significance between groups. , significance relative to control.
