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BioMed Research International has retracted this article. The article was found to contain images with signs of duplication and manipulation in Figures 1(a), 1(b), 1(d), 2(b), 3(a), 3(b), 3(c), 3(d), 3(k), 4(d), 4(g), 4(m), 4(p), 8, 10(c), 10(d), 10(e), 10(f), 10(g), 10(h), 10(i), 10(j), 10(k), 10(l), and 10(o) and duplication from Talukdar D. An induced glutathione-deficient mutant in grass pea (Lathyrus sativus L.): Modifications in plant morphology, alteration in antioxidant activities and increased sensitivity to cadmium. Biorem. Biodiv Bioavail. 2012; 6: 75–86 in Figure 2B and from Dibyendu Talukdar and Tulika Talukdar, “Superoxide-Dismutase Deficient Mutants in Common Beans (Phaseolus vulgaris L.): Genetic Control, Differential Expressions of Isozymes, and Sensitivity to Arsenic,” BioMed Research International, vol. 2013, Article ID 782450, 11 pages, 2013, doi: 10.1155/2013/782450 in Figure 10.

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  1. D. Talukdar and T. Talukdar, “Leaf rolling and stem fasciation in grass pea (Lathyrus sativus l.) mutant are mediated through glutathione-dependent cellular and metabolic changes and associated with a metabolic diversion through cysteine during phenotypic reversal,” BioMed Research International, vol. 2014, Article ID 479180, 21 pages, 2014.
BioMed Research International
Volume 2014, Article ID 479180, 21 pages
Research Article

Leaf Rolling and Stem Fasciation in Grass Pea (Lathyrus sativus L.) Mutant Are Mediated through Glutathione-Dependent Cellular and Metabolic Changes and Associated with a Metabolic Diversion through Cysteine during Phenotypic Reversal

1Department of Botany, R.P.M College, University of Calcutta, Uttarpara, Hooghly, West Bengal 712 258, India
2Department of Botany, Acharya Prafulla Chandra Roy Government College, University of North Bengal, Darjeeling, West Bengal 734 010, India

Received 25 February 2014; Accepted 24 April 2014; Published 28 May 2014

Academic Editor: Pavel Hozak

Copyright © 2014 Dibyendu Talukdar and Tulika Talukdar. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


A Lathyrus sativus L. mutant isolated in ethylmethane sulfonate-treated M2 progeny of mother variety BioL-212 and designated as rlfL-1 was characterized by inwardly rolled-leaf and stem and bud fasciations. The mutant exhibited karyomorphological peculiarities in both mitosis and meiosis with origin of aneuploidy. The mitosis was vigorous with high frequency of divisional cells and their quick turnover presumably steered cell proliferations. Significant transcriptional upregulations of cysteine and glutathione synthesis and concomitant stimulations of glutathione-mediated antioxidant defense helped rlfL-1 mutant to maintain balanced reactive oxygen species (ROS) metabolisms, as deduced by ROS-imaging study. Glutathione synthesis was shut down in buthionine sulfoximine- (BSO-) treated mother plant and mutant, and leaf-rolling and stems/buds fasciations in the mutant were reversed, accompanied by normalization of mitotic cell division process. Antioxidant defense was downregulated under low glutathione-redox but cysteine-desulfurations and photorespiratory glycolate oxidase transcripts were markedly overexpressed, preventing cysteine overaccumulation but resulted in excess H2O2 in BSO-treated mutant. This led to oxidative damage in proliferating cells, manifested by severe necrosis in rolled-leaf and fasciated stems. Results indicated vital role of glutathione in maintaining abnormal proliferations in plant organs, and its deficiency triggered phenotypic reversal through metabolic diversions of cysteine and concomitant cellular and metabolic modulations.