Methylamine-Sensitive Amperometric Biosensor Based on (His)₆-Tagged Hansenula polymorpha Methylamine Oxidase Immobilized on the Gold Nanoparticles

Table 1

The activity of AMO during immobilization on the nAu surfaces.

	Enzyme preparation	Supernatant after enzyme immobilization	Immobilized AMO on nAu
𝑉, mL	0.03	0.015	0.02
𝐶protein, mg·mL^−1	0.75	0.52	*
Totalprotein, mg	0.02	0.08	*
𝐴, U·mL^−1	40.1 ± 1.1	2.55 ± 0.12	35.5 ± 0.2
Total 𝐴, U	1.2 ± 0.1	0.04 ± 0.002	0.71 ± 0.01
Yield (%)	=100	3.3 ± 0.1	59.2 ± 1.2

∗Under sensitivity.