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BioMed Research International
Volume 2014, Article ID 480545, 13 pages
http://dx.doi.org/10.1155/2014/480545
Research Article

Preliminary In Vitro and In Vivo Evaluation of Antidiabetic Activity of Ducrosia anethifolia Boiss. and Its Linear Furanocoumarins

1Natural Compounds Chemistry Department, National Research Centre, Dokki, Giza 12622, Egypt
2Therapeutic Chemistry Department, National Research Centre, Dokki, Giza 12622, Egypt
3University of Tabuk, Tabuk 71491, Saudi Arabia
4Chemistry Department, Faculty of Science, King Khalid University, Abha 9004, Saudi Arabia
5Faculté de Pharmacie de Toulouse, Laboratoire des IMRCP UMR CNRS 5623, Université de Toulouse, Université Paul-Sabatier, 118 Route de Narbonne, 31062 Toulouse, France

Received 13 December 2013; Revised 28 January 2014; Accepted 20 February 2014; Published 31 March 2014

Academic Editor: Vickram Ramkumar

Copyright © 2014 Nagwa M. M. Shalaby et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aim. Ducrosia anethifolia is used as flavoring additive. There have been little detailed phytochemical reports on this genus and the antidiabetic activity of this plant is not yet evaluated. Method. Structure of compounds was deduced by spectroscopic analyses. Preliminary in vitro evaluation of the antidiabetic activity of crude extract and its furanocoumarins was carried out (α-amylase, α-glucosidase, and β-galactosidase). The in vivo activity was investigated by measuring some oxidative stress markers. Biomarkers of liver injury and kidney were also determined. Results. Eight linear furanocoumarins, psoralen, 5-methoxypsoralen, 8-methoxypsoralen, imperatorin, isooxypeucedanin, pabulenol, oxypeucedanin methanolate, oxypeucedanin hydrate, and 3-O-glucopyranosyl-β-sitosterol, were isolated. All compounds were reported for the first time from the genus Ducrosia except pabulenol. The blood glucose level, liver function enzymes, total protein, lipid, and cholesterol levels were significantly normalized by extract treatment. The antioxidant markers, glucolytic, and gluconeogenic enzymes were significantly ameliorated and the elevated level of kidney biomarkers in the diabetic groups was restored. The compounds showed inhibitory activity in a concentration dependant manner. Imperatorin and 5-methoxypsoralen showed the most potent inhibiting power. Conclusion. D. anethifolia extract showed hypoglycemic, hypolipidemic, and antioxidant effect as well as ameliorating kidney function. This extract and some linear furanocoumarins exhibited carbohydrate metabolizing enzymes inhibitory effect.