BioMed Research International / 2014 / Article / Fig 4

Research Article

Antisecretory Effect of Hydrogen Sulfide on Gastric Acid Secretion and the Involvement of Nitric Oxide

Figure 4

(a) Effect of NaHS on the gastric mucosal mRNA expression of endothelial nitric oxide synthase (eNOS). Analysis of semiquantitative real-time PCR results showed that the administration of NaHS increased the mRNA expression of eNOS. C: control, NaHS: animals received NaHS (80 μg/kg, i.v.) just prior to stimulation of the gastric acid secretion, NaHS + L-NAME: animals received L-NAME at 10 mg/kg, i.v., 5 min before the administration of NaHS (80 μg/kg, i.v.), and PAG: rats received cystathionine-γ-lyase inhibitor, propargylglycine (PAG), at 50 mg/kg, i.v. concomitant with the gastric distention. (b) animals received normal saline at 2 mL/kg, i.p. (C: control), or L-cysteine at 50 mg/kg, i.p. (L-cys), once a day for five days before stimulation of the gastric acid secretion. Asterisks indicate a significant increase ( ) as compared to the control group; indicates a significant decrease as compared with the control group. Data are expressed as mean ± S.E.M.