Antisecretory Effect of Hydrogen Sulfide on Gastric Acid Secretion and the Involvement of Nitric Oxide
(a) Effect of NaHS on the gastric mucosal mRNA expression of endothelial nitric oxide synthase (eNOS). Analysis of semiquantitative real-time PCR results showed that the administration of NaHS increased the mRNA expression of eNOS. C: control, NaHS: animals received NaHS (80 μg/kg, i.v.) just prior to stimulation of the gastric acid secretion, NaHS + L-NAME: animals received L-NAME at 10 mg/kg, i.v., 5 min before the administration of NaHS (80 μg/kg, i.v.), and PAG: rats received cystathionine-γ-lyase inhibitor, propargylglycine (PAG), at 50 mg/kg, i.v. concomitant with the gastric distention. (b) animals received normal saline at 2 mL/kg, i.p. (C: control), or L-cysteine at 50 mg/kg, i.p. (L-cys), once a day for five days before stimulation of the gastric acid secretion. Asterisks indicate a significant increase () as compared to the control group; indicates a significant decrease as compared with the control group. Data are expressed as mean ± S.E.M.