Antisecretory Effect of Hydrogen Sulfide on Gastric Acid Secretion and the Involvement of Nitric Oxide
Effect of NaHS (a) and L-cysteine (b) on the gastric mucosal mRNA expression of H+/K+-ATPase α-subunit. (a) Analysis of semiquantitative real-time PCR results showed that the administration of NaHS decreased the mRNA expression of H+/K+-ATPase α-subunit. In contrast, this level increased in PAG-treated rats as compared to the control group. C: control, NaHS: animals received NaHS (80 μg/kg, i.v.) just prior to stimulation of the gastric acid secretion, NaHS + L-NAME: animals received L-NAME at 10 mg/kg, i.v., 5 min before the administration of NaHS (80 μg/kg, i.v.), and PAG: rats received cystathionine-γ-lyase inhibitor, propargylglycine (PAG), at 50 mg/kg, i.v. concomitant with the gastric distention. (b) Animals received normal saline at 2 mL/kg, i.p. (C: control), or L-cysteine at 50 mg/kg, i.p. (L-cys), once a day for five days before stimulation of the gastric acid secretion. Asterisks indicate a significant decrease () as compared to the control group; in the above column indicates a significant increase () as compared to the control. Data are expressed as mean ± S.E.M.