A Disintegrin and Metalloproteinase 9 Is Involved in Ectodomain Shedding of Receptor-Binding Cancer Antigen Expressed on SiSo Cells
Figure 1
Change in RCAS1 expression and shedding after gene transfection. (a) ADAM9 siRNA transfection in SiSo cells. (A) A Western blot revealed that ADAM9 expression diminished after ADAM9 siRNA transfection. (B) Flow cytometric analysis showed that cell surface expression of RCAS1 increased after ADAM9 siRNA transfection. (C) Transfection of ADAM9 siRNA significantly augmented RCAS1 expression but decreased the RCAS1 concentration in culture supernatants (). (b) ADAM9 cDNA transfection in MCF-7 cells. (A) A Western blot revealed that ADAM9 expression was enhanced after ADAM9 cDNA transfection. (B) Flow cytometric analysis showed that the cell surface expression of RCAS1 decreased after ADAM9 cDNA transfection. (C) Transfection of ADAM9 cDNA significantly diminished RCAS1 expression but increased the concentration of RCAS1 in culture supernatants (). Mean values of triplicate measurements are shown.
