Quantitative Evaluation of E1 Endoglucanase Recovery from Tobacco Leaves Using the Vacuum Infiltration-Centrifugation Method
Figure 1
(a) A schematic of the cross-section of a tobacco leaf. Arrows represent the bulk flow of the apoplast wash fluid through the leaf. The flow is in the direction of the centrifugal force as fluid is forced out through the stoma. (b) The centrifugation step to recover AWF from this Nicotiana benthamiana leaf was interrupted. The bulk flow of the infiltrated fluid moves evenly through the leaf over time in the direction of the centrifugal force (arrow).
