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BioMed Research International
Volume 2014, Article ID 487535, 8 pages
http://dx.doi.org/10.1155/2014/487535
Research Article

Strontium Promotes Cementoblasts Differentiation through Inhibiting Sclerostin Expression In Vitro

1Department of Orthodontics, School of Stomatology, Jilin University, 1500 Qinghua Road, Changchun, Jilin 130021, China
2Institute of Virology and AIDS Research, First Hospital, Jilin University, Changchun 130021, China

Received 2 January 2014; Revised 13 March 2014; Accepted 12 April 2014; Published 9 June 2014

Academic Editor: Kunikazu Tsuji

Copyright © 2014 Xingfu Bao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cementogenesis, performed by cementoblasts, is important for the repair of root resorption caused by orthodontic treatment. Based on recent studies, strontium has been applied for osteoporosis treatment due to its positive effect on osteoblasts. Although promising, the effect of strontium on cementoblasts is still unclear. So the aim of this research was to clarify and investigate the effect of strontium on cementogenesis via employing cementoblasts as model. A series of experiments including MTT, alkaline phosphatase activity, gene analysis, alizarin red staining, and western blot were carried out to evaluate the proliferation and differentiation of cementoblasts. In addition, expression of sclerostin was checked to analyze the possible mechanism. Our results show that strontium inhibits the proliferation of cementoblasts with a dose dependent manner; however, it can promote the differentiation of cementoblasts via downregulating sclerostin expression. Taking together, strontium may facilitate cementogenesis and benefit the treatment of root resorption at a low dose.