Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous Clearance

Table 2

Primers used for SYBR Green PCR based amplification of TLRs in this study.

Toll receptors	Primers	Sequence	NCBI accession reference number	Start site	Stop site	Product size (bp)
TLR 3	Forward	5′-GTG CCA GAA ACT TCC CAT GT-3′	NM_003265.2	383	402	231
	Reverse	5′-TCC AGC TGA ACC TGA GTT CC-3′		594	613
TLR 7	Forward	5′-AAT GTC ACA GCC GTC CCT AC-3′	NM_016562.3	782	801	223
	Reverse	5′-GCG CAT CAA AAG CAT TTA CA-3′		985	1004
TLR 8	Forward	5′-TGT GAT GGT GGT GCT TCA AT-3′	XM_005274543.2	1028	1047	187
	Reverse	5′-ATG CCC CAG AGG CTA TTT CT-3′		1214	1195

Binding site refers to the site where polymerase chain reaction (PCR) primer binds to prime duplication of a complement to the existing complementary DNA (cDNA) sequence of the mRNA; bp: base pair.