BioMed Research International / 2014 / Article / Tab 2

Research Article

Modulation of TLR 3, 7 and 8 Expressions in HCV Genotype 3 Infected Individuals: Potential Correlations of Pathogenesis and Spontaneous Clearance

Table 2

Primers used for SYBR Green PCR based amplification of TLRs in this study.

Toll receptorsPrimersSequenceNCBI accession reference numberStart siteStop siteProduct size (bp)

TLR 3Forward5′-GTG CCA GAA ACT TCC CAT GT-3′NM_003265.2383402231
Reverse5′-TCC AGC TGA ACC TGA GTT CC-3′594613

TLR 7Forward5′-AAT GTC ACA GCC GTC CCT AC-3′NM_016562.3782801223
Reverse5′-GCG CAT CAA AAG CAT TTA CA-3′9851004

TLR 8Forward5′-TGT GAT GGT GGT GCT TCA AT-3′XM_005274543.210281047187
Reverse5′-ATG CCC CAG AGG CTA TTT CT-3′12141195

Binding site refers to the site where polymerase chain reaction (PCR) primer binds to prime duplication of a complement to the existing complementary DNA (cDNA) sequence of the mRNA; bp: base pair.