Table 1: Designed primers for tetraprimer ARMS-PCR reactions, annealing temperature, and fragments sizes.

SNPPrimer sequenceAnnealing temperature (°C)Fragments length

XRCC1 Arg194Trp rs1799782FO: 5′-CGTCCCAGGTAAGCTGTAC-3′ 
RO: 5′-CACTCCTATCTATGGGACACAG-3′ 
FI: 5′-CGGGGGCTCTCTTCTTCATCC-3′ 
RI: 5′-CACCTGGGGATGTCTTGTTGATACA-3′
63Outer: 471
Arg: 297
Trp: 219

XRCC1 Arg399Gln rs25487FO: 5′-ACCAGCTGTGCCTTTGCCAACACC-3′ 
RO: 5′-CTGGAGTACCCCAGCCCCTGCC-3′ 
FI: 5′-GTCGGCGGCTGCCCTCACA-3′ 
RI: 5′-TGGCGTGTGAGGCCTTACCACC-3′
68Outer: 283
Arg: 183
Gln: 140