Human Schwann Cells Seeded on a Novel Collagen-Based Microstructured Nerve Guide Survive, Proliferate, and Modify Neurite Outgrowth
Figure 2
HSC cultures after propagation in serum-containing medium contained approximately 60% Schwann cells (S100β/vimentin positive) and 40% fibroblasts (S100β negative/vimentin positive (scale bar 200 μm), white arrows. (a) vimentin. (c) S100β. (e) Merge with DAPI nuclear staining. MACS purification method resulted in Schwann cell cultures of approximately >98% purity (b), (d), and (f), scale bar 500 μm. Cell purity was maintained along the culture period as well as after cell seeding onto the collagen scaffolds.
