Human Schwann Cells Seeded on a Novel Collagen-Based Microstructured Nerve Guide Survive, Proliferate, and Modify Neurite Outgrowth

Figure 7

TPSLM images of hSCs seeded onto Perimaix S2 prototypes at 3 ((a), (d), (g), and (j)), 7 ((b), (e), (h), and (k)), and 14 ((c), (f), (i), and (l)) days after seeding. (a), (b), and (c): S100β; (d), (e), and (f): vimentin; (g), (h), and (i): collagen structure visualized by SHG; (j), (k). (l) Merge, scale bar 50 μm. Surface analysis of the scaffolds demonstrated many hSCs that clustered in bundles orienting along the microchannels. Note in blue the clear length orientation (channel-orientation indicated by the white arrow) of the microchannels, with collagen interconnections to provide stability.

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