Human Schwann Cells Seeded on a Novel Collagen-Based Microstructured Nerve Guide Survive, Proliferate, and Modify Neurite Outgrowth
Figure 9
Neurite outgrowth of primary DRG sensory neurons treated with conditioned medium of hSC cultivated on Perimaix. ((a), (b)) In culture DRG sensory neurons adopt primarily two phenotypes: elongated (a) or arborised (b) [11]. (c) Normal culture medium (negative control, nC) did not induce neurite outgrowth. (e) hSCs cultivated on Perimaix released soluble factors that induced neurite outgrowth in rat DRG neurons to a similar extent as when neurons were treated with conditioned medium derived from hSC grown in a normal culture plate, (positive control pC, (d)).
