Mechanical Stress Stimulates the Osteo/Odontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 1/2 and JNK MAPK Pathways
Figure 3
ALP assay and alizarin red staining of SCAPs. (a) Mechanical stress group presented a higher level () of ALP activity than control group after 3-day and 5-day cultures, respectively. (b) After 14-day and 21-day cultures, calcium contents in mechanical stress group were significantly elevated () as compared with those in control group. Values are described as the , . (c) After 21-day culture, alizarin red staining demonstrated that mechanical stress group generated more calcification nodules than control group. (d) ALP analyses were performed to test the effects of different magnitudes of stress (0 g, 200 g, 250 g. and 300 g) at different time points (6 h, 12 h, 24 h, and 48 h) on SCAPs. Values were described as the , , .
