Mechanical Stress Stimulates the Osteo/Odontoblastic Differentiation of Human Stem Cells from Apical Papilla via ERK 1/2 and JNK MAPK Pathways
Figure 5
Effects of mechanical stress on MAPK pathway of SCAPs. (a) Protein expressions of phosphor-ERK 1/2 and ERK 1/2 by Western blot. (b) Protein expressions of phosphor-JNK and JNK. (c) Protein expressions of phosphor-p38 and p38. (d) The ratio of pERK/ERK intensity in (a). (e) The ratio of pJNK/JNK intensity in (b). (f) The ratio of pP-38/P38 intensity in (c). (g) Protein expressions of c-Fos and c-Jun by Western blot. (h) The ratio of p-c-Fos/c-Fos and p-c-Jun/c-Jun intensity in (g). (i) MTT analysis of SCAPs under the treatment of stress and/or inhibitors. (j) ALP analysis SCAPs under the treatment of stress and/or inhibitors. (k), (l) Real-time RT-PCR results of the gene expression (RUNX2, OSX, OCN, and DSPP) in inhibitors-treated SCAPs, respectively, at day 3 (k) and day 7 (l). Values are described as the , . .
